Testing for Lyme

Lyme  and Microbial Testing

Upon consultation we can work together  to ensure you have the right pathology investigations and testing that are implicated in Lyme, Vector or Tick born disease.  There are many Immune or inflammatory markers that can be affected, so looking at these as well as pathogenic load and doing a Lyme or Microbial specific test is important for an overall health examination.

Available Lyme test kits and Pathology Forms at Botanical Health

ImmunoScience test (American Lab) – Lyme multi-peptide Igm and IgG Elisa Assay. Cost $880

Includes B. burgdorferi, B.b garinii, B. afzelii, Babesia, Echrlichia and Bartonella

 

Australian Biologics Lyme test (Australian Test)

Serology
EuroLine Blot Borrelia IgM/IgG $220

Immunofluorescence (IIFA/IFA)
Bartonella IgM/IgG $140

Chemiluminescence (CLIA)
$140 per test:

Borrelia IgM/IgG
Mycoplasma pneumoniae IgM/IgG
Toxoplasmosis IgM/IgG
C.pneumoniae IgA/IgM
Brucella IgM/IgG

Tick-Borne Encephalitis IgM/IgG (Coming Soon)

Immunology

EliSpot Borrelia B31/OSP​ $300

Basic Panel $600

  • EuroLine Blot – Borrelia

  • CLIA – M.pneumoniae

  • CLIA – C.pneumoniae

  • IFA – Bartonella

Comprehensive Panel $999

  • EuroLine Blot – Borrelia

  • CLIA – Borrelia

  • CLIA – M.pneumoniae

  • CLIA – C.pneumoniae

  • EliSpot – Borrelia

  • IFA Bartonella

Australian Rickettsial Reference Laboratories (Australian Test)

  • Rickettsia: This includes the screening for rickettsia of the spotted fever group (SFG), typhus group (TG) and scrub typhus group (STG).  Although STG infections are caused by the genus Orientia, these organisms are closely related to the rickettsia and hence are found in the same cluster. There are three tests offered for the diagnosis of these diseases, namely, an Indirect microimmunofluorescence assay (IFA), polymerase chain reaction (PCR) and culture.  For the IFA, Clotted blood (serum) may be submitted for serological diagnosis. If possible a follow-up specimen should be sent a minimum of 7-10 days later to detect a change from negative to a positive antibody level (seroconversion) or if the first sample is positive, a significant increase in antibody levels will also confirm the diagnosis. The PCR involves the detection of a specific DNA targets of the bacterial genome.  The culture assay involves the isolation of the organism in cell culture and can take up to six weeks.  Cost $58.75
  • Q fever: Q fever is caused by the organism Coxiella burnetii. There are three tests offered for the diagnosis of these diseases, namely, an Indirect microimmunofluorescence assay (IFA), polymerase chain reaction (PCR) and culture.  For the IFA, Clotted blood (serum) may be submitted for serological diagnosis. If possible a follow-up specimen should be sent a minimum of 7-10 days later to detect a change from negative to a positive antibody level (seroconversion) or if the first sample is positive, a significant increase in antibody levels will also confirm the diagnosis. Q-fever serology involves testing the patient’s serum against phase 2 C. burnetii ( acute Q-fever) and phase 1 C. burnetii (chronic Q-fever).  The PCR involves the detection of 2 specific DNA targets of the C. burnetii genome.  The culture assay involves the isolation of the organism in cell culture and can take up to six weeks.Cost $58.75
  • Lyme Disease: Lyme disease is caused by Borrelia species and is tick transmitted.  The laboratory offers serology, polymerase chain reaction (PCR) and culture for Lyme disease diagnosis.  For the serology, serum is examined using three tests for the presence of specific antibody.  The laboratory uses 2 primary screening assays, indirect microimmunofluorescence (IFA) and the enzyme linked immunosorbent assay (ELISA).  A third test called the immunoblot is used for confirmatory purposes.   PCR is used to directly identify Borrelia species DNA and cultures attempt to isolate the causative organisms and can tale up to six weeks. Cost $122.40
  • Neoehrlichia: Neoehlichia species have been detected in 12.9% of Ixodes holocyclus ticks from Australia.  Although Neoehlichia species in the northern hemisphere have been shown to cause diseases their pathogeniicity in Australia is currently unknown.  As there is no cell cultured isolate for this agent the only diagnostic test that can be offered at this time is PCR.
  • Anaplasma / Ehrlichia: These diseases are both tick transmitted and although there is evidence that some species exist in Australia the main human pathogens Anaplasma phagocytophilum and Ehrlichia chafeensis are yet to be identified in Australia.  For these diseases the laboratory offers IFA, PCR and culture.  Their IFA is specific for the above mentioned pathogens, however their PCR will detect most members of these genuses. The culture assays are designed to isolate most of these species. Cost $24.70
  • Babesia: Babesia species are not bacteria, they are in fact parasites that can be transmitted by tick bite.  These agents have been reported from Australia.  The laboratory is currently offering an IFA  for the detection of this agent.  Cost $24.70
  • Bartonella: Bartonella bacteria cause several diseases in humans. The two most common agents are B. henselae (cat scratch disease) and B. quintana (Trench fever). The laboratory is currently offering an IFA for the detection of these agents.  Cost $24.70
  • Whipples Disease: Whipple’s disease is a raresystemic infectious disease caused by the bacterium Tropheryma whipplei.  Commonly considered a gastrointestinal disorder, Whipple’s disease primarily causes malabsorption. This laboratory offers PCR and an  IFA test.

Upon consultation we can work together to ensure you have the appropriate pathology investigation and testing that is specific for you, Lyme, tick born disease.